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A three-dimensional movie of structural changes in bacteriorhodopsin.

Nango E, Royant A, Kubo M, Nakane T, Wickstrand C, Kimura T, Tanaka T, Tono K, Song C, Tanaka R, Arima T, Yamashita A, Kobayashi J, Hosaka T, Mizohata E, Nogly P, Sugahara M, Nam D, Nomura T, Shimamura T, Im D, Fujiwara T, Yamanaka Y, Jeon B, Nishizawa T, Oda K, Fukuda M, Andersson R, Båth P, Dods R, Davidsson J, Matsuoka S, Kawatake S, Murata M, Nureki O, Owada S, Kameshima T, Hatsui T, Joti Y, Schertler G, Yabashi M, Bondar AN, Standfuss J, Neutze R, Iwata S
Science 354 1552-1557 (2016)
Related entries: 5b6v, 5b6x, 5b6y, 5b6z, 5h2h, 5h2i, 5h2j, 5h2k, 5h2l, 5h2m, 5h2n, 5h2o, 5h2p

Cited: 143 times
EuropePMC logo PMID: 28008064

Abstract

Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to milliseconds following photoactivation. An initially twisted retinal chromophore displaces a conserved tryptophan residue of transmembrane helix F on the cytoplasmic side of the protein while dislodging a key water molecule on the extracellular side. The resulting cascade of structural changes throughout the protein shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient.

Articles - 5b6w mentioned but not cited (2)

  1. Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance. Enkavi G, Javanainen M, Kulig W, Róg T, Vattulainen I. Chem Rev 119 5607-5774 (2019)
  2. A tool for visualizing protein motions in time-resolved crystallography. Wickstrand C, Katona G, Nakane T, Nogly P, Standfuss J, Nango E, Neutze R. Struct Dyn 7 024701 (2020)


Reviews citing this publication (41)

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