› 5b8h

X-ray diffraction
2.2Å resolution

Crystal Structure of Type III pantothenate kinase (PanK III) from Burkholderia cenocepacia complexed with pantothenate, imidodiphosphate, and AMP

Released:
DOI: 10.2210/pdb5b8h/pdb
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PDB entry 5b8h coloured by chain, front view.
PDB entry 5b8h coloured by chain, side view.
PDB entry 5b8h coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type III pantothenate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 30.75 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B4E9P3 (Residues: 1-266; Coverage: 100%)
Gene names: BCAL0693, BceJ2315_06870, coaX
Sequence domains: Type III pantothenate kinase
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

4 bound ligands:
Ligand 2PN 2 x 2PN
Ligand PAU 2 x PAU
Ligand EDO 2 x EDO
Ligand AMP 1 x AMP
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 44.21Å b: 72.75Å c: 154.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.228
Expression system: Escherichia coli BL21(DE3)

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