5bkn

X-ray diffraction
3Å resolution

Crystallographic structure of a cubic crystal form of STMV (84.5 degree rotation) grown from chloride

Released:
Primary publication:
Structures of additional crystal forms of Satellite tobacco mosaic virus grown from a variety of salts.
Acta Crystallogr F Struct Biol Commun 77 473-483 (2021)
PMID: 34866603

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero 117-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-538660 (preferred)
Entry contents:
1 distinct polypeptide molecule
10 distinct RNA molecules
Macromolecules (11 distinct):
Coat protein Chains: A, B, C, D, E, F, G, GG, H, HH, I, II, J, JJ, K, KK, L, M, N, O
Molecule details ›
Chains: A, B, C, D, E, F, G, GG, H, HH, I, II, J, JJ, K, KK, L, M, N, O
Length: 159 amino acids
Theoretical weight: 17.53 KDa
Source organism: Satellite tobacco mosaic virus
UniProt:
  • Canonical: P17574 (Residues: 1-159; Coverage: 100%)
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*A)-3') Chains: P, S, a
Molecule details ›
Chains: P, S, a
Length: 8 nucleotides
Theoretical weight: 2.59 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*AP*AP*AP*AP*AP*A)-3') Chain: T
Molecule details ›
Chain: T
Length: 6 nucleotides
Theoretical weight: 1.93 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chain: V
Molecule details ›
Chain: V
Length: 9 nucleotides
Theoretical weight: 2.92 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chains: TT, UU, X, Y
Molecule details ›
Chains: TT, UU, X, Y
Length: 10 nucleotides
Theoretical weight: 3.25 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') Chains: e, qq
Molecule details ›
Chains: e, qq
Length: 12 nucleotides
Theoretical weight: 3.63 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*U)-3') Chain: h
Molecule details ›
Chain: h
Length: 7 nucleotides
Theoretical weight: 2.1 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*UP*UP*UP*UP*UP*U)-3') Chains: i, ll
Molecule details ›
Chains: i, ll
Length: 6 nucleotides
Theoretical weight: 1.79 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') Chains: WW, kk, m
Molecule details ›
Chains: WW, kk, m
Length: 9 nucleotides
Theoretical weight: 2.71 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*U)-3') Chain: n
Molecule details ›
Chain: n
Length: 8 nucleotides
Theoretical weight: 2.4 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chain: bb
Molecule details ›
Chain: bb
Length: 12 nucleotides
Theoretical weight: 3.91 KDa
Source organism: Satellite tobacco mosaic virus

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P23
Unit cell:
a: 234.65Å b: 234.65Å c: 234.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.255