› 5bmh

X-ray diffraction
1.6Å resolution

Nitroxide Spin Labels in Protein GB1: T44 Mutant, Crystal Form B

Released:
DOI: 10.2210/pdb5bmh/pdb
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PDB entry 5bmh coloured by chain, front view.
PDB entry 5bmh coloured by chain, side view.
PDB entry 5bmh coloured by chain, top view.
Source organism: Streptococcus sp. 'group G'
Primary publication:
Rotameric preferences of a protein spin label at edge-strand β-sheet sites.
Cunningham TF, Pornsuwan S, Horne WS, Saxena S
Protein Sci 25 1049-60 (2016)
PMID: 26948069

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 56 amino acids
Theoretical weight: 6.23 KDa
Source organism: Streptococcus sp. 'group G'
Expression system: Escherichia coli
UniProt:
  • Canonical: P19909 (Residues: 304-357; Coverage: 10%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

1 bound ligand:
Ligand MTN 1 x MTN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 25.037Å b: 37.194Å c: 49.217Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.175
Expression system: Escherichia coli

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Citations

7 citation in other articles

Benchmark Test and Guidelines for DEER/PELDOR Experiments on Nitroxide-Labeled Biomolecules.
Schiemann et al. (2021)
6 more

2 mentions without citation

Rotameric preferences of a protein spin label at edge-strand β-sheet sites.
Cunningham et al. (2016)
1 more

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