› 5c1f

X-ray diffraction
2.36Å resolution

Structure of the Imp2 F-BAR domain

Released:
DOI: 10.2210/pdb5c1f/pdb
PDB entry 5c1f coloured by chain, front view.
PDB entry 5c1f coloured by chain, side view.
PDB entry 5c1f coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
The Tubulation Activity of a Fission Yeast F-BAR Protein Is Dispensable for Its Function in Cytokinesis.
McDonald NA, Takizawa Y, Feoktistova A, Xu P, Ohi MD, Vander Kooi CW, Gould KL
OpenAccess logo Cell Rep 14 534-546 (2016)
PMID: 26776521

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171213 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Septation protein imp2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 310 amino acids
Theoretical weight: 35.96 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q10199 (Residues: 15-320; Coverage: 46%)
Gene names: SPBC11C11.02, imp2
Sequence domains: Fes/CIP4, and EFC/F-BAR homology domain

Ligands and Environments

1 bound ligand:
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM, APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 187.337Å b: 33.802Å c: 120.765Å
α: 90° β: 122.54° γ: 90°
R-values:
R R work R free
0.193 0.19 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Linking up at the BAR: Oligomerization and F-BAR protein function.
McDonald et al. (2016)
4 more

4 mentions without citation

Spatial arrangement of proteins in planar and curved membranes by PPM 3.0.
Lomize et al. (2022)
3 more