› 5c6j

X-ray diffraction
2.1Å resolution

Crystal Structure of Gadolinium derivative of HEWL solved using Free-Electron Laser radiation

Released:
DOI: 10.2210/pdb5c6j/pdb
PDB entry 5c6j coloured by chain, front view.
PDB entry 5c6j coloured by chain, side view.
PDB entry 5c6j coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Towards phasing using high X-ray intensity.
Galli L, Son SK, Barends TR, White TA, Barty A, Botha S, Boutet S, Caleman C, Doak RB, Nanao MH, Nass K, Shoeman RL, Timneanu N, Santra R, Schlichting I, Chapman HN
OpenAccess logo IUCrJ 2 627-34 (2015)
PMID: 26594370

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

3 bound ligands:
Ligand DO3 2 x DO3
Ligand CL 4 x CL
Ligand GD 2 x GD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: P43212
Unit cell:
a: 79.2Å b: 79.2Å c: 39.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.272

Quick links

Citations

2 review citations

An outlook on using serial femtosecond crystallography in drug discovery.
Mishin et al. (2019)
1 more