› 5c7r

X-ray diffraction
1.94Å resolution

Revealing surface waters on an antifreeze protein by fusion protein crystallography

Released:
DOI: 10.2210/pdb5c7r/pdb
Model geometry
Fit model/data
PDB entry 5c7r coloured by chain, front view.
PDB entry 5c7r coloured by chain, side view.
PDB entry 5c7r coloured by chain, top view.
Source organism: Zoarces americanus
Primary publication:
Revealing Surface Waters on an Antifreeze Protein by Fusion Protein Crystallography Combined with Molecular Dynamic Simulations.
Sun T, Gauthier SY, Campbell RL, Davies PL
J Phys Chem B 119 12808-15 (2015)
PMID: 26371748

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 444 amino acids
Theoretical weight: 48.51 KDa
Source organism: Zoarces americanus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-384; Coverage: 97%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 48.05Å b: 110.8Å c: 96.57Å
α: 90° β: 94.16° γ: 90°
R-values:
R R work R free
0.178 0.176 0.234
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Water Determines the Structure and Dynamics of Proteins.
Bellissent-Funel et al. (2016)
1 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO