› 5c7s

X-ray diffraction
2.1Å resolution

PRPP complexed with two Mn2+ in the active site of Mycobacterium tuberculosis anthranilate phosphoribosyltransferase (AnPRT; trpD)

Released:
DOI: 10.2210/pdb5c7s/pdb
PDB entry 5c7s coloured by chain, front view.
PDB entry 5c7s coloured by chain, side view.
PDB entry 5c7s coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Evans GL, Baker EN, Lott JS, TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Reaction catalysed: 
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-551851 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anthranilate phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 379 amino acids
Theoretical weight: 38.95 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WFX5 (Residues: 2-370; Coverage: 100%)
Gene names: MTCY190.03c, Rv2192c, trpD
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand PRP 2 x PRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 94.178Å b: 78.481Å c: 100.446Å
α: 90° β: 109.9° γ: 90°
R-values:
R R work R free
0.205 0.202 0.254
Expression system: Escherichia coli BL21(DE3)

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