› 5ccj

X-ray diffraction
1.65Å resolution

Crystal structure of the quintuple mutant of the synaptotagmin-1 C2B domain

Released:
DOI: 10.2210/pdb5ccj/pdb
PDB entry 5ccj coloured by chain, front view.
PDB entry 5ccj coloured by chain, side view.
PDB entry 5ccj coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Architecture of the synaptotagmin-SNARE machinery for neuronal exocytosis.
Zhou Q, Lai Y, Bacaj T, Zhao M, Lyubimov AY, Uervirojnangkoorn M, Zeldin OB, Brewster AS, Sauter NK, Cohen AE, Soltis SM, Alonso-Mori R, Chollet M, Lemke HT, Pfuetzner RA, Choi UB, Weis WI, Diao J, Südhof TC, Brunger AT
OpenAccess logo Nature 525 62-7 (2015)
PMID: 26280336

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-149380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Synaptotagmin-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 152 amino acids
Theoretical weight: 17.02 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21707 (Residues: 271-421; Coverage: 36%)
Gene name: Syt1
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

2 bound ligands:
Ligand GOL 27 x GOL
Ligand SO4 22 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 68.92Å b: 107.59Å c: 110.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.15 0.178
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

43 review citations

The Mechanisms and Functions of Synaptic Facilitation.
Jackman et al. (2017)
42 more

4 mentions without citation

Architecture of the synaptotagmin-SNARE machinery for neuronal exocytosis.
Zhou et al. (2015)
3 more