5cr2

X-ray diffraction
2.9Å resolution

E. coli MazF in complex with single strand DNA substrate analog

Released:
Model geometry
Fit model/data

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-113166 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
Endoribonuclease toxin MazF Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 117 amino acids
Theoretical weight: 12.94 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE70 (Residues: 1-111; Coverage: 100%)
Gene names: JW2753, b2782, chpA, chpAK, mazF
Sequence domains: PemK-like, MazF-like toxin of type II toxin-antitoxin system
Structure domains: SH3 type barrels.
ssDNA substrate analog Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 7 nucleotides
Theoretical weight: 2.08 KDa
Source organism: Escherichia virus MS2
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P3121
Unit cell:
a: 113.96Å b: 113.96Å c: 47.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.223 0.29
Expression systems:
  • Escherichia coli
  • Not provided