› 5crf

X-ray diffraction
1.8Å resolution

Structure of the penicillin-binding protein PonA1 from Mycobacterium Tuberculosis

Released:
DOI: 10.2210/pdb5crf/pdb
PDB entry 5crf coloured by chain, front view.
PDB entry 5crf coloured by chain, side view.
PDB entry 5crf coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystal structures of the transpeptidase domain of the Mycobacterium tuberculosis penicillin-binding protein PonA1 reveal potential mechanisms of antibiotic resistance.
Filippova EV, Kieser KJ, Luan CH, Wawrzak Z, Kiryukhina O, Rubin EJ, Anderson WF
OpenAccess logo FEBS J 283 2206-18 (2016)
PMID: 27101811

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-159814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-binding protein 1A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 430 amino acids
Theoretical weight: 44.64 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P71707 (Residues: 391-820; Coverage: 52%)
Gene names: MTCY21D4.13, Rv0050, ponA1
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

1 bound ligand:
Ligand PO4 5 x PO4
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 46.006Å b: 333.314Å c: 47.528Å
α: 90° β: 108.37° γ: 90°
R-values:
R R work R free
0.163 0.161 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Cell wall peptidoglycan in Mycobacterium tuberculosis: An Achilles' heel for the TB-causing pathogen.
Maitra et al. (2019)
3 more

1 mention without citation

Crystal structures of the transpeptidase domain of the Mycobacterium tuberculosis penicillin-binding protein PonA1 reveal potential mechanisms of antibiotic resistance.
Filippova et al. (2016)