› 5d09

X-ray diffraction
2.35Å resolution

Neisseria meningitidis 3 deoxy-D-arabino-heptulosonate 7-phosphate synthase Phe211Ala variant

Released:
DOI: 10.2210/pdb5d09/pdb
PDB entry 5d09 coloured by chain, front view.
PDB entry 5d09 coloured by chain, side view.
PDB entry 5d09 coloured by chain, top view.
Source organism: Neisseria meningitidis MC58
Entry authors: Heyes LC, Parker EJ

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-575498 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-2-dehydro-3-deoxyheptonate aldolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 351 amino acids
Theoretical weight: 38.64 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K169 (Residues: 1-351; Coverage: 100%)
Gene names: NMB0307, aroG
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

5 bound ligands:
Ligand MN 4 x MN
Ligand PEP 1 x PEP
Ligand TMO 1 x TMO
Ligand PEG 2 x PEG
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 78.675Å b: 135.727Å c: 149.356Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.259
Expression system: Escherichia coli

Quick links