5d1x

X-ray diffraction
3.21Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-212097 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
D4-30 Light Chain Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 24.09 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
D4-30 Heavy Chain Chain: B
Molecule details ›
Chain: B
Length: 259 amino acids
Theoretical weight: 27.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
P5 Heavy Chain Chain: C
Molecule details ›
Chain: C
Length: 274 amino acids
Theoretical weight: 29.43 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
P5 Light Chain Chain: D
Molecule details ›
Chain: D
Length: 216 amino acids
Theoretical weight: 23.65 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Iron-regulated surface determinant protein B Chain: E
Molecule details ›
Chain: E
Length: 118 amino acids
Theoretical weight: 13.88 KDa
Source organism: Staphylococcus aureus subsp. aureus USA300
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q2FZF0 (Residues: 341-458; Coverage: 20%)
Gene names: SAOUHSC_01079, frpB, isdB, sasJ, sirH
Sequence domains: Iron Transport-associated domain
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P41
Unit cell:
a: 111.251Å b: 111.251Å c: 105.226Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.269 0.31
Expression systems:
  • Homo sapiens
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'