› 5d2f

X-ray diffraction
1.74Å resolution

4-oxalocrotonate decarboxylase from Pseudomonas putida G7 - apo form

Released:
DOI: 10.2210/pdb5d2f/pdb
PDB entry 5d2f coloured by chain, front view.
PDB entry 5d2f coloured by chain, side view.
PDB entry 5d2f coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Crystal Structures of Apo and Liganded 4-Oxalocrotonate Decarboxylase Uncover a Structural Basis for the Metal-Assisted Decarboxylation of a Vinylogous β-Keto Acid.
Guimarães SL, Coitinho JB, Costa DM, Araújo SS, Whitman CP, Nagem RA
Biochemistry 55 2632-45 (2016)
PMID: 27082660

Function and Biology Details

Reaction catalysed: 
(3E)-2-oxohex-3-enedioate = 2-oxopent-4-enoate + CO(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarylacetoacetase-like C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 30.75 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1XGK3 (Residues: 1-264; Coverage: 100%)
Gene name: nahK
Sequence domains: Fumarylacetoacetate (FAA) hydrolase family
Structure domains: Fumarylacetoacetase-like, C-terminal domain

Ligands and Environments

3 bound ligands:
Ligand EDO 12 x EDO
Ligand CL 2 x CL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: P212121
Unit cell:
a: 43.763Å b: 44.939Å c: 125.141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The fumarylacetoacetate hydrolase (FAH) superfamily of enzymes: multifunctional enzymes from microbes to mitochondria.
Weiss et al. (2018)
1 other article

1 mention without citation

Crystal Structures of Apo and Liganded 4-Oxalocrotonate Decarboxylase Uncover a Structural Basis for the Metal-Assisted Decarboxylation of a Vinylogous β-Keto Acid.
Guimarães et al. (2016)