› 5d3o

X-ray diffraction
2.79Å resolution

Crystal structure of full length human glutaminase C expressed in E.coli

Released:
DOI: 10.2210/pdb5d3o/pdb
PDB entry 5d3o coloured by chain, front view.
PDB entry 5d3o coloured by chain, side view.
PDB entry 5d3o coloured by chain, top view.
Source organism: Homo sapiens
Entry author: Huang Q

Function and Biology Details

Reaction catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131797 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminase kidney isoform, mitochondrial 65 kDa chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 527 amino acids
Theoretical weight: 58.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O94925 (Residues: 72-550; Coverage: 72%)
  • Best match: O94925-3 (Residues: 72-598)
Gene names: GLS, GLS1, KIAA0838
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Unit cell:
a: 51.609Å b: 139.371Å c: 179.789Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.266
Expression system: Escherichia coli BL21(DE3)

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