› 5d4u

X-ray diffraction
2Å resolution

SAM-bound HcgC from Methanocaldococcus jannaschii

Released:
DOI: 10.2210/pdb5d4u/pdb
PDB entry 5d4u coloured by chain, front view.
PDB entry 5d4u coloured by chain, side view.
PDB entry 5d4u coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Identification of HcgC as a SAM-Dependent Pyridinol Methyltransferase in [Fe]-Hydrogenase Cofactor Biosynthesis.
Fujishiro T, Bai L, Xu T, Xie X, Schick M, Kahnt J, Rother M, Hu X, Ermler U, Shima S
Angew Chem Int Ed Engl 55 9648-51 (2016)
PMID: 27391308

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized protein MJ0489 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 268 amino acids
Theoretical weight: 30.57 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57913 (Residues: 1-268; Coverage: 100%)
Gene name: MJ0489
Sequence domains: FeGP cofactor biosynthesis protein, methyltransferase HcgC

Ligands and Environments

2 bound ligands:
Ligand SAM 4 x SAM
Ligand SO4 16 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 86.29Å b: 70.52Å c: 91.23Å
α: 90° β: 105.21° γ: 90°
R-values:
R R work R free
0.2 0.198 0.228
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Heterologous Hydrogenase Overproduction Systems for Biotechnology-An Overview.
Fan et al. (2020)
4 other articles