› 5db4

X-ray diffraction
2.28Å resolution

Mycobacterium abscessus NadD in complex with Mg-ATP, space group I41

Released:
DOI: 10.2210/pdb5db4/pdb
PDB entry 5db4 coloured by chain, front view.
PDB entry 5db4 coloured by chain, side view.
PDB entry 5db4 coloured by chain, top view.
Source organism: Mycobacteroides abscessus ATCC 19977
Entry authors: Reed RW, Korotkov KV, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + beta-nicotinate-D-ribonucleotide = diphosphate + deamido-NAD(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-109380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable nicotinate-nucleotide adenylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 23.51 KDa
Source organism: Mycobacteroides abscessus ATCC 19977
Expression system: Escherichia coli
UniProt:
  • Canonical: B1MMZ4 (Residues: 2-211; Coverage: 100%)
Gene names: MAB_1621, nadD
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I41
Unit cell:
a: 67.098Å b: 67.098Å c: 229.538Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.248
Expression system: Escherichia coli

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