5dcv

X-ray diffraction
3.4Å resolution

Crystal structure of PhoRpp38-SL12M complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-158537 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Large ribosomal subunit protein eL8 Chains: A, C
Molecule details ›
Chains: A, C
Length: 132 amino acids
Theoretical weight: 14.65 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62009 (Residues: 1-123; Coverage: 100%)
Gene names: PH1496, rpl7ae
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
RNA (47-MER) Chains: B, D
Molecule details ›
Chains: B, D
Length: 51 nucleotides
Theoretical weight: 16.77 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 59.09Å b: 79.17Å c: 98.47Å
α: 90° β: 107.4° γ: 90°
R-values:
R R work R free
0.265 0.263 0.311
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided