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Crystal structures in an anti-HIV antibody lineage from immunization of Rhesus macaques

X-ray diffraction
2.488Å resolution
PDB entry 5dd0 coloured by chain, front view.
PDB entry 5dd0 coloured by chain, side view.
PDB entry 5dd0 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Transmembrane protein gp41 and IG-heavy chain and IG-light kappa chain
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Multimeric state: hetero trimer
Accessible surface area: 19973.75 Å2
Buried surface area: 4797.15 Å2
Dissociation area: 538.93 Å2
Dissociation energy (ΔGdiss): 2.98 kcal/mol
Dissociation entropy (TΔSdiss): 7.7 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-229838

Macromolecules

ANTI-HIV ANTIBODY DH570 FAB HEAVY CHAIN
Chain: H
Length: 233 amino acids
Theoretical weight: 24.78 KDa
Source organism: Macaca mulatta
Expression system: Homo sapiens
InterPro:
CATH: Immunoglobulins
ANTI-HIV ANTIBODY DH570 FAB HEAVY CHAIN in PDB entry 5dd0, assembly 1, front view.
ANTI-HIV ANTIBODY DH570 FAB HEAVY CHAIN in PDB entry 5dd0, assembly 1, side view.
ANTI-HIV ANTIBODY DH570 FAB HEAVY CHAIN in PDB entry 5dd0, assembly 1, top view.
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ANTI-HIV ANTIBODY DH570 FAB HEAVY LIGHT
Chain: L
Length: 214 amino acids
Theoretical weight: 23.08 KDa
Source organism: Macaca mulatta
Expression system: Homo sapiens
InterPro:
CATH: Immunoglobulins
ANTI-HIV ANTIBODY DH570 FAB HEAVY LIGHT in PDB entry 5dd0, assembly 1, front view.
ANTI-HIV ANTIBODY DH570 FAB HEAVY LIGHT in PDB entry 5dd0, assembly 1, side view.
ANTI-HIV ANTIBODY DH570 FAB HEAVY LIGHT in PDB entry 5dd0, assembly 1, top view.
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Transmembrane protein gp41
Chain: P
Length: 13 amino acids
Theoretical weight: 1.4 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: P04578 (Residues: 660-670; Coverage: 1%)
Gene name: env
Transmembrane protein gp41 in PDB entry 5dd0, assembly 1, front view.
Transmembrane protein gp41 in PDB entry 5dd0, assembly 1, side view.
Transmembrane protein gp41 in PDB entry 5dd0, assembly 1, top view.
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