› 5de5

X-ray diffraction
3Å resolution

Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA.

Released:
DOI: 10.2210/pdb5de5/pdb
PDB entry 5de5 coloured by chain, front view.
PDB entry 5de5 coloured by chain, side view.
PDB entry 5de5 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure reveals specific recognition of a G-quadruplex RNA by a β-turn in the RGG motif of FMRP.
Vasilyev N, Polonskaia A, Darnell JC, Darnell RB, Patel DJ, Serganov A
Proc Natl Acad Sci U S A 112 E5391-400 (2015)
PMID: 26374839

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-554902 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Fragile X messenger ribonucleoprotein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 18 amino acids
Theoretical weight: 1.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q06787 (Residues: 527-544; Coverage: 3%)
Gene name: FMR1
sc1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 35 nucleotides
Theoretical weight: 11.46 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand K 7 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21212
Unit cell:
a: 57.06Å b: 130.01Å c: 36.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.221
Expression system: Not provided

Quick links

Citations

29 review citations

RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview.
Šponer et al. (2018)
28 more

7 mentions without citation

The emerging structural complexity of G-quadruplex RNAs.
Banco et al. (2021)
6 more