› 5dn9

X-ray diffraction
1.5Å resolution

Crystal structure of Candida boidinii formate dehydrogenase complexed with NAD+ and azide

Released:
DOI: 10.2210/pdb5dn9/pdb
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PDB entry 5dn9 coloured by chain, front view.
PDB entry 5dn9 coloured by chain, side view.
PDB entry 5dn9 coloured by chain, top view.
Source organism: [Candida] boidinii
Primary publication:
Structural and Kinetic Studies of Formate Dehydrogenase from Candida boidinii.
Guo Q, Gakhar L, Wickersham K, Francis K, Vardi-Kilshtain A, Major DT, Cheatum CM, Kohen A
Biochemistry 55 2760-71 (2016)
PMID: 27100912

Function and Biology Details

Reaction catalysed: 
Formate + NAD(+) = CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100800 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 364 amino acids
Theoretical weight: 40.36 KDa
Source organism: [Candida] boidinii
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0A1EQY0 (Residues: 1-364; Coverage: 100%)
Gene names: Cboi02_000553900, FDH
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
Ligand NAD 2 x NAD
Ligand AZI 2 x AZI
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21
Unit cell:
a: 50.931Å b: 116.617Å c: 63.208Å
α: 90° β: 106.9° γ: 90°
R-values:
R R work R free
0.14 0.138 0.169
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

3 review citations

Improving the Enzymatic Cascade of Reactions for the Reduction of CO2 to CH3OH in Water: From Enzymes Immobilization Strategies to Cofactor Regeneration and Cofactor Suppression.
Di Spiridione et al. (2022)
2 more

11 mentions without citation

Structural and Kinetic Studies of Formate Dehydrogenase from Candida boidinii.
Guo et al. (2016)
10 more

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