› 5dp2

X-ray diffraction
0.96Å resolution

CurF ER cyclopropanase from curacin A biosynthetic pathway

Released:
DOI: 10.2210/pdb5dp2/pdb
PDB entry 5dp2 coloured by chain, front view.
PDB entry 5dp2 coloured by chain, side view.
PDB entry 5dp2 coloured by chain, top view.
Source organism: Lyngbya majuscula
Primary publication:
Structural Basis for Cyclopropanation by a Unique Enoyl-Acyl Carrier Protein Reductase.
Khare D, Hale WA, Tripathi A, Gu L, Sherman DH, Gerwick WH, Håkansson K, Smith JL
Structure 23 2213-2223 (2015)
PMID: 26526850

Function and Biology Details

Reaction catalysed: 
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179532 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CurF Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 38.22 KDa
Source organism: Lyngbya majuscula
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6DNE7 (Residues: 273-611; Coverage: 11%)
Gene name: curF
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 108.906Å b: 47.294Å c: 76.902Å
α: 90° β: 123.44° γ: 90°
R-values:
R R work R free
0.117 0.117 0.131
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The architectures of iterative type I PKS and FAS.
Herbst et al. (2018)
3 more

7 mentions without citation

Structural Basis for Cyclopropanation by a Unique Enoyl-Acyl Carrier Protein Reductase.
Khare et al. (2015)
6 more