› 5dpg

X-ray diffraction
1.85Å resolution

sfGFP mutant - 133 p-cyano-L-phenylalanine

Released:
DOI: 10.2210/pdb5dpg/pdb
PDB entry 5dpg coloured by chain, front view.
PDB entry 5dpg coloured by chain, side view.
PDB entry 5dpg coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Probing the effectiveness of spectroscopic reporter unnatural amino acids: a structural study.
Dippel AB, Olenginski GM, Maurici N, Liskov MT, Brewer SH, Phillips-Piro CM
Acta Crystallogr D Struct Biol 72 121-30 (2016)
PMID: 26894540

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.96 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand EDO 10 x EDO
Ligand NA 2 x NA
2 modified residues:
Ligand CRO 1 x CRO
Ligand 4CF 1 x 4CF

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21212
Unit cell:
a: 60.774Å b: 68.057Å c: 53.268Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.149 0.192
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Measuring Electric Fields in Biological Matter Using the Vibrational Stark Effect of Nitrile Probes.
Slocum et al. (2018)
7 other articles

3 mentions without citation

Probing the effectiveness of spectroscopic reporter unnatural amino acids: a structural study.
Dippel et al. (2016)
2 more