› 5dqm

X-ray diffraction
1.3Å resolution

Green/cyan WasCFP at pH 2.0

Released:
DOI: 10.2210/pdb5dqm/pdb
Model geometry
Fit model/data
PDB entry 5dqm coloured by chain, front view.
PDB entry 5dqm coloured by chain, side view.
PDB entry 5dqm coloured by chain, top view.
Source organism: Aequorea coerulescens
Primary publication:
Crystal structure of pH and T dependent green fluorescent protein WasCFP with Trp based chromophore
Pletnev VZ, Pletneva N, Efremov RG, Goryacheva EA, Artemyev IV, Arkhipova SF, Sarkisyan KS, Mishin AS, Lukyanov KA, Pletnev SV
Russ. J. Bioorganic Chem. 42 (6) (2016)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 28.06 KDa
Source organism: Aequorea coerulescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRF 1 x CRF

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 47.268Å b: 51.528Å c: 47.529Å
α: 90° β: 97.7° γ: 90°
R-values:
R R work R free
0.163 0.162 0.208
Expression system: Escherichia coli

Quick links

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO