› 5ds1

X-ray diffraction
2.63Å resolution

Core domain of the class II small heat-shock protein HSP 17.7 from Pisum sativum

Released:
DOI: 10.2210/pdb5ds1/pdb
PDB entry 5ds1 coloured by chain, front view.
PDB entry 5ds1 coloured by chain, side view.
PDB entry 5ds1 coloured by chain, top view.
Source organism: Pisum sativum
Primary publication:
Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions.
Hochberg GKA, Shepherd DA, Marklund EG, Santhanagoplan I, Degiacomi MT, Laganowsky A, Allison TM, Basha E, Marty MT, Galpin MR, Struwe WB, Baldwin AJ, Vierling E, Benesch JLP
Science 359 930-935 (2018)
PMID: 29472485

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
17.1 kDa class II heat shock protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 95 amino acids
Theoretical weight: 10.65 KDa
Source organism: Pisum sativum
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P19242 (Residues: 43-135; Coverage: 61%)
Gene name: HSP17.7
Sequence domains: Hsp20/alpha crystallin family
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: C2
Unit cell:
a: 48.86Å b: 82.69Å c: 88.9Å
α: 90° β: 92.11° γ: 90°
R-values:
R R work R free
0.237 0.231 0.297
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

10 review citations

Small heat shock proteins: Simplicity meets complexity.
Haslbeck et al. (2019)
9 more

1 mention without citation

The Common Bean Small Heat Shock Protein Nodulin 22 from Phaseolus vulgaris L. Assembles into Functional High-Molecular-Weight Oligomers.
Fernández-Silva et al. (2022)