› 5du8

X-ray diffraction
2.23Å resolution

Function and Biology Details

Reaction catalysed: 
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-552300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable enoyl-CoA hydratase EchA6 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 263 amino acids
Theoretical weight: 28.23 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WNP1 (Residues: 1-243; Coverage: 100%)
Gene names: MTCY31.33, Rv0905, echA6
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains: 2-enoyl-CoA Hydratase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand 5FK 3 x 5FK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 51.39Å b: 115.98Å c: 171.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Mycobacterial cell wall biosynthesis: a multifaceted antibiotic target.
Abrahams et al. (2018)
9 more