› 5dx0

X-ray diffraction
2.05Å resolution

Crystal structure of CARM1, sinefungin, and H3 peptide (R17)

Released:
DOI: 10.2210/pdb5dx0/pdb
PDB entry 5dx0 coloured by chain, front view.
PDB entry 5dx0 coloured by chain, side view.
PDB entry 5dx0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Insights into Ternary Complex Formation of Human CARM1 with Various Substrates.
Boriack-Sjodin PA, Jin L, Jacques SL, Drew A, Sneeringer C, Scott MP, Moyer MP, Ribich S, Moradei O, Copeland RA
ACS Chem Biol 11 763-71 (2016)
PMID: 26551522

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-551322 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone-arginine methyltransferase CARM1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 349 amino acids
Theoretical weight: 39.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q86X55 (Residues: 134-479; Coverage: 57%)
Gene names: CARM1, PRMT4
Sequence domains:
Structure domains:
Histone H3.3 Chains: F, G, H, I
Molecule details ›
Chains: F, G, H, I
Length: 20 amino acids
Theoretical weight: 1.91 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P84243 (Residues: 14-31; Coverage: 13%)
Gene names: H3-3A, H3-3B, H3.3A, H3.3B, H3F3, H3F3A, H3F3B, PP781

Ligands and Environments

3 bound ligands:
Ligand SFG 4 x SFG
Ligand GOL 2 x GOL
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 75.722Å b: 98.847Å c: 208.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.211 0.263
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

5 review citations

Protein arginine methylation: from enigmatic functions to therapeutic targeting.
Wu et al. (2021)
4 more

6 mentions without citation

A chemical probe of CARM1 alters epigenetic plasticity against breast cancer cell invasion.
Cai et al. (2019)
5 more