› 5e27

X-ray diffraction
2.6Å resolution

The structure of Resuscitation Promoting Factor B from M. tuberculosis reveals unexpected ubiquitin-like domains

Released:
DOI: 10.2210/pdb5e27/pdb
PDB entry 5e27 coloured by chain, front view.
PDB entry 5e27 coloured by chain, side view.
PDB entry 5e27 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
The structure of Resuscitation promoting factor B from M. tuberculosis reveals unexpected ubiquitin-like domains.
Ruggiero A, Squeglia F, Romano M, Vitagliano L, De Simone A, Berisio R
Biochim Biophys Acta 1860 445-51 (2016)
PMID: 26549874

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Resuscitation-promoting factor RpfB Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 25.9 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WG29 (Residues: 115-362; Coverage: 73%)
Gene names: MTC1237.26, Rv1009, rpfB
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2221
Unit cell:
a: 102.376Å b: 126.99Å c: 86.286Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.291
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Lytic transglycosylases: concinnity in concision of the bacterial cell wall.
Dik et al. (2017)
5 more

3 mentions without citation

A Structural View at Vaccine Development against M. tuberculosis.
Romano et al. (2023)
2 more