› 5e40

X-ray diffraction
2.05Å resolution

3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase from Mycobacterium tuberculosis with D-tyrosine bound in the phenylalanine binding site

Released:
DOI: 10.2210/pdb5e40/pdb
PDB entry 5e40 coloured by chain, front view.
PDB entry 5e40 coloured by chain, side view.
PDB entry 5e40 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Probing the Sophisticated Synergistic Allosteric Regulation of Aromatic Amino Acid Biosynthesis in Mycobacterium tuberculosis Using ᴅ-Amino Acids.
Reichau S, Blackmore NJ, Jiao W, Parker EJ
OpenAccess logo PLoS One 11 e0152723 (2016)
PMID: 27128682

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-520545 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-2-dehydro-3-deoxyheptonate aldolase AroG Chains: A, B
Molecule details ›
Chains: A, B
Length: 464 amino acids
Theoretical weight: 50.83 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: O53512 (Residues: 1-462; Coverage: 100%)
Gene names: Rv2178c, aroG
Sequence domains: Class-II DAHP synthetase family

Ligands and Environments

4 bound ligands:
Ligand SO4 5 x SO4
Ligand MN 2 x MN
Ligand DTY 2 x DTY
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P3221
Unit cell:
a: 205.451Å b: 205.451Å c: 66.803Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.194 0.207
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Recent advancements in the development of anti-tuberculosis drugs.
Chetty et al. (2017)
1 more

1 mention without citation

Probing the Sophisticated Synergistic Allosteric Regulation of Aromatic Amino Acid Biosynthesis in Mycobacterium tuberculosis Using ᴅ-Amino Acids.
Reichau et al. (2016)