› 5e7g

X-ray diffraction
2.37Å resolution

Crystal structure of Bacova_02650 with xylogluco-oligosaccharide

Released:
DOI: 10.2210/pdb5e7g/pdb
PDB entry 5e7g coloured by chain, front view.
PDB entry 5e7g coloured by chain, side view.
PDB entry 5e7g coloured by chain, top view.
Source organism: Bacteroides ovatus ATCC 8483
Primary publication:
Molecular Dissection of Xyloglucan Recognition in a Prominent Human Gut Symbiont.
Tauzin AS, Kwiatkowski KJ, Orlovsky NI, Smith CJ, Creagh AL, Haynes CA, Wawrzak Z, Brumer H, Koropatkin NM
OpenAccess logo mBio 7 e02134-15 (2016)
PMID: 27118585

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-501183 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
IPT/TIG domain-containing protein BACOVA_02650 Chain: A
Molecule details ›
Chain: A
Length: 456 amino acids
Theoretical weight: 49.65 KDa
Source organism: Bacteroides ovatus ATCC 8483
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A7LXT4 (Residues: 34-489; Coverage: 99%)
Gene name: BACOVA_02650
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, XYS
Carbohydrate polymer
3 bound ligands:
Ligand CA 1 x CA
Ligand SO4 4 x SO4
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: R32
Unit cell:
a: 207.39Å b: 207.39Å c: 117.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.198 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Introduction to the human gut microbiota.
Thursby et al. (2017)
6 more

2 mentions without citation

Cell Surface Xyloglucan Recognition and Hydrolysis by the Human Gut Commensal Bacteroides uniformis.
Grondin et al. (2022)
1 more