› 5ena

X-ray diffraction
1.35Å resolution

Xray crystal structure of isotope-labeled human insulin

Released:
DOI: 10.2210/pdb5ena/pdb
Model geometry
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PDB entry 5ena coloured by chain, front view.
PDB entry 5ena coloured by chain, side view.
PDB entry 5ena coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallization of Enantiomerically Pure Proteins from Quasi-Racemic Mixtures: Structure Determination by X-Ray Diffraction of Isotope-Labeled Ester Insulin and Human Insulin.
Mandal K, Dhayalan B, Avital-Shmilovici M, Tokmakoff A, Kent SB
Chembiochem 17 421-5 (2016)
PMID: 26707939

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-134182 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I213
Unit cell:
a: 77.935Å b: 77.935Å c: 77.935Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.164
Expression system: Not provided

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Citations

4 citation in other articles

Chiral Inactivation: An Old Phenomenon with a New Twist.
Raskatov JA. (2017)
3 more

5 mentions without citation

D-Peptide and D-Protein Technology: Recent Advances, Challenges, and Opportunities.
Lander et al. (2023)
4 more

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