› 5eou

X-ray diffraction
2.4Å resolution

Pseudomonas aeruginosa PilM:PilN1-12 bound to ATP

Released:
DOI: 10.2210/pdb5eou/pdb
PDB entry 5eou coloured by chain, front view.
PDB entry 5eou coloured by chain, side view.
PDB entry 5eou coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
PilN Binding Modulates the Structure and Binding Partners of the Pseudomonas aeruginosa Type IVa Pilus Protein PilM.
McCallum M, Tammam S, Little DJ, Robinson H, Koo J, Shah M, Calmettes C, Moraes TF, Burrows LL, Howell PL
J Biol Chem 291 11003-15 (2016)
PMID: 27022027

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type IV pilus inner membrane component PilM; Type IV pilus inner membrane component PilN Chains: A, B
Molecule details ›
Chains: A, B
Length: 372 amino acids
Theoretical weight: 40.04 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: G3XD28 (Residues: 1-354; Coverage: 100%)
  • Canonical: G3XD30 (Residues: 1-12; Coverage: 6%)
Gene names: PA5043, PA5044, pilM, pilN
Sequence domains: Type IV pilus assembly protein PilM;

Ligands and Environments

5 bound ligands:
Ligand SO4 8 x SO4
Ligand CL 4 x CL
Ligand NA 2 x NA
Ligand MG 2 x MG
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P321
Unit cell:
a: 131.79Å b: 131.79Å c: 78.993Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.212 0.239
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Recent perspectives on the molecular basis of biofilm formation by Pseudomonas aeruginosa and approaches for treatment and biofilm dispersal.
Skariyachan et al. (2018)
2 more

3 mentions without citation

PilN Binding Modulates the Structure and Binding Partners of the Pseudomonas aeruginosa Type IVa Pilus Protein PilM.
McCallum et al. (2016)
2 more