› 5euu

X-ray diffraction
1.87Å resolution

Rat prestin STAS domain in complex with chloride

Released:
DOI: 10.2210/pdb5euu/pdb
PDB entry 5euu coloured by chain, front view.
PDB entry 5euu coloured by chain, side view.
PDB entry 5euu coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
The STAS domain of mammalian SLC26A5 prestin harbours an anion-binding site.
Lolli G, Pasqualetto E, Costanzi E, Bonetto G, Battistutta R
Biochem J 473 365-70 (2016)
PMID: 26635354

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prestin Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 15.77 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9EPH0 (Residues: 505-563, 637-718; Coverage: 19%)
Gene names: Pres, Slc26a5
Sequence domains: STAS domain
Structure domains: STAS domain

Ligands and Environments

4 bound ligands:
Ligand CL 2 x CL
Ligand EDO 3 x EDO
Ligand PG4 1 x PG4
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P3121
Unit cell:
a: 62.133Å b: 62.133Å c: 66.587Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.157 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Chansporter complexes in cell signaling.
Abbott GW. (2017)
1 more

2 mentions without citation

Cryo-EM structures and functional characterization of murine Slc26a9 reveal mechanism of uncoupled chloride transport.
Walter et al. (2019)
1 more