› 5ex8

X-ray diffraction
2.1Å resolution

Structure of P450 StaF from glycopeptide antibiotic A47934 biosynthesis; ethylene glycol cryo

Released:
DOI: 10.2210/pdb5ex8/pdb
PDB entry 5ex8 coloured by chain, front view.
PDB entry 5ex8 coloured by chain, side view.
PDB entry 5ex8 coloured by chain, top view.
Source organism: Streptomyces toyocaensis
Primary publication:
Biochemical and structural characterisation of the second oxidative crosslinking step during the biosynthesis of the glycopeptide antibiotic A47934.
Ulrich V, Brieke C, Cryle MJ
OpenAccess logo Beilstein J Org Chem 12 2849-2864 (2016)
PMID: 28144358

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-569188 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chain: A
Molecule details ›
Chain: A
Length: 424 amino acids
Theoretical weight: 47.34 KDa
Source organism: Streptomyces toyocaensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KLL7 (Residues: 1-391; Coverage: 100%)
Gene name: BU52_01275
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand EDO 16 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 110.05Å b: 110.05Å c: 93.65Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Unrivalled diversity: the many roles and reactions of bacterial cytochromes P450 in secondary metabolism.
Greule et al. (2018)
2 other articles

4 mentions without citation

Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function.
Rudolf et al. (2017)
3 more