› 5exp

X-ray diffraction
1.8Å resolution

AAA+ domain of FleQ from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5exp/pdb
PDB entry 5exp coloured by chain, front view.
PDB entry 5exp coloured by chain, side view.
PDB entry 5exp coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Mechanistic insights into c-di-GMP-dependent control of the biofilm regulator FleQ from Pseudomonas aeruginosa.
Matsuyama BY, Krasteva PV, Baraquet C, Harwood CS, Sondermann H, Navarro MV
Proc Natl Acad Sci U S A 113 E209-18 (2016)
PMID: 26712005

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124382 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator FleQ Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.3 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3XCV0 (Residues: 137-394; Coverage: 53%)
Gene names: PA1097, fleQ
Sequence domains: Sigma-54 interaction domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P65
Unit cell:
a: 104.907Å b: 104.907Å c: 42.752Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.168 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Cyclic di-GMP: second messenger extraordinaire.
Jenal et al. (2017)
20 more

4 mentions without citation

Mechanistic insights into c-di-GMP-dependent control of the biofilm regulator FleQ from Pseudomonas aeruginosa.
Matsuyama et al. (2016)
3 more