› 5f42

X-ray diffraction
2.06Å resolution

Activity and Crystal Structure of Francisella novicida UDP-N-Acetylglucosamine Acyltransferase

Released:
DOI: 10.2210/pdb5f42/pdb
PDB entry 5f42 coloured by chain, front view.
PDB entry 5f42 coloured by chain, side view.
PDB entry 5f42 coloured by chain, top view.
Source organism: Francisella tularensis subsp. novicida U112
Primary publication:
Crystal structure and activity of Francisella novicida UDP-N-acetylglucosamine acyltransferase.
Joo SH, Chung HS
Biochem Biophys Res Commun 478 1223-9 (2016)
PMID: 27545601

Function and Biology Details

Reaction catalysed: 
A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = an [acyl-carrier-protein] + a UDP-3-O-(3-hydroxyacylyl)-N-acetyl-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine acyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 280 amino acids
Theoretical weight: 30.53 KDa
Source organism: Francisella tularensis subsp. novicida U112
Expression system: Escherichia coli
UniProt:
  • Canonical: A0Q7Y0 (Residues: 1-259; Coverage: 100%)
Gene names: FTN_1478, lpxA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: R32
Unit cell:
a: 105.38Å b: 105.38Å c: 285.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.21 0.26
Expression system: Escherichia coli

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Citations

1 review citation

Genetic Determinants of Antibiotic Resistance in Francisella.
Kassinger et al. (2021)

1 mention without citation

Crystal structure and activity of Francisella novicida UDP-N-acetylglucosamine acyltransferase.
Joo et al. (2016)