› 5f7l

X-ray diffraction
2.74Å resolution

Blood group antigen binding adhesin BabA of Helicobacter pylori strain 17875 in complex with Nanobody Nb-ER14

Released:
DOI: 10.2210/pdb5f7l/pdb
PDB entry 5f7l coloured by chain, front view.
PDB entry 5f7l coloured by chain, side view.
PDB entry 5f7l coloured by chain, top view.
Source organisms:
Primary publication:
Structural Insights into Polymorphic ABO Glycan Binding by Helicobacter pylori.
Moonens K, Gideonsson P, Subedi S, Bugaytsova J, Romaõ E, Mendez M, Nordén J, Fallah M, Rakhimova L, Shevtsova A, Lahmann M, Castaldo G, Brännström K, Coppens F, Lo AW, Ny T, Solnick JV, Vandenbussche G, Oscarson S, Hammarström L, Arnqvist A, Berg DE, Muyldermans S, Borén T, Remaut H
Cell Host Microbe 19 55-66 (2016)
PMID: 26764597

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208101 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SabA N-terminal extracellular adhesion domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 466 amino acids
Theoretical weight: 49.37 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: O52269 (Residues: 45-480; Coverage: 61%)
Gene name: babA2
Sequence domains: SabA N-terminal extracellular adhesion domain
Nanobody Nb-ER14 Chains: B, D
Molecule details ›
Chains: B, D
Length: 129 amino acids
Theoretical weight: 14.19 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Unit cell:
a: 42.402Å b: 133.715Å c: 201.623Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.265
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Pathogenesis of Helicobacter pylori infection.
Backert et al. (2016)
22 more

4 mentions without citation

Predicted structural mimicry of spike receptor-binding motifs from highly pathogenic human coronaviruses.
Beaudoin et al. (2021)
3 more