› 5f7o

X-ray diffraction
1.81Å resolution

Crystal structure of Mutant Q289L of adenosine/Methylthioadenosine phosphorylase from Schistosoma mansoni in complex with Adenine

Released:
DOI: 10.2210/pdb5f7o/pdb
PDB entry 5f7o coloured by chain, front view.
PDB entry 5f7o coloured by chain, side view.
PDB entry 5f7o coloured by chain, top view.
Source organism: Schistosoma mansoni
Primary publication:
Crystal Structure of Schistosoma mansoni Adenosine Phosphorylase/5'-Methylthioadenosine Phosphorylase and Its Importance on Adenosine Salvage Pathway.
Torini JR, Brandão-Neto J, DeMarco R, Pereira HD
OpenAccess logo PLoS Negl Trop Dis 10 e0005178 (2016)
PMID: 27935959

Function and Biology Details

Reaction catalysed: 
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-methyl-5'-thioadenosine phosphorylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 320 amino acids
Theoretical weight: 35.24 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I0B503 (Residues: 1-299; Coverage: 100%)
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand ADE 2 x ADE
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 74.354Å b: 81.873Å c: 81.76Å
α: 90° β: 101.95° γ: 90°
R-values:
R R work R free
0.184 0.182 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Pyrimidine metabolism in schistosomes: A comparison with other parasites and the search for potential chemotherapeutic targets.
El Kouni MH. (2017)
3 other articles

1 mention without citation

Crystal Structure of Schistosoma mansoni Adenosine Phosphorylase/5'-Methylthioadenosine Phosphorylase and Its Importance on Adenosine Salvage Pathway.
Torini et al. (2016)