› 5fa1

X-ray diffraction
2.1Å resolution

The structure of the beta-3-deoxy-D-manno-oct-2-ulosonic acid transferase domain of WbbB

Released:
DOI: 10.2210/pdb5fa1/pdb
PDB entry 5fa1 coloured by chain, front view.
PDB entry 5fa1 coloured by chain, side view.
PDB entry 5fa1 coloured by chain, top view.
Source organism: Raoultella terrigena
Primary publication:
Bacterial β-Kdo glycosyltransferases represent a new glycosyltransferase family (GT99).
Ovchinnikova OG, Mallette E, Koizumi A, Lowary TL, Kimber MS, Whitfield C
Proc Natl Acad Sci U S A 113 E3120-9 (2016)
PMID: 27199480

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyltransferase 99 N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 410 amino acids
Theoretical weight: 46.1 KDa
Source organism: Raoultella terrigena
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6U8B0 (Residues: 2-401; Coverage: 36%)
Gene name: wbbB
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand C5P 2 x C5P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2221
Unit cell:
a: 92.75Å b: 159.39Å c: 120.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Lipopolysaccharide O-antigens-bacterial glycans made to measure.
Whitfield et al. (2020)
3 more

2 mentions without citation

Bacterial β-Kdo glycosyltransferases represent a new glycosyltransferase family (GT99).
Ovchinnikova et al. (2016)
1 more