› 5fie

X-ray diffraction
2Å resolution

Crystal structure of N-terminal domain of shaft pilin spaA from Lactobacillus rhamnosus GG

Released:
DOI: 10.2210/pdb5fie/pdb
PDB entry 5fie coloured by chain, front view.
PDB entry 5fie coloured by chain, side view.
PDB entry 5fie coloured by chain, top view.
Source organism: Lacticaseibacillus rhamnosus GG
Primary publication:
New insights about pilus formation in gut-adapted Lactobacillus rhamnosus GG from the crystal structure of the SpaA backbone-pilin subunit.
Chaurasia P, Pratap S, von Ossowski I, Palva A, Krishnan V
OpenAccess logo Sci Rep 6 28664 (2016)
PMID: 27349405

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell surface protein SpaA Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 16.72 KDa
Source organism: Lacticaseibacillus rhamnosus GG
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P41212
Unit cell:
a: 88.615Å b: 88.615Å c: 48.892Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.222
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Novel Molecular Insights about Lactobacillar Sortase-Dependent Piliation.
von Ossowski I. (2017)
2 more

2 mentions without citation

New insights about pilus formation in gut-adapted Lactobacillus rhamnosus GG from the crystal structure of the SpaA backbone-pilin subunit.
Chaurasia et al. (2016)
1 more