› 5flx

Electron Microscopy
3.9Å resolution

Function and Biology Details

Reaction catalysed: 
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 35-mer (preferred)
PDBe Complex ID:
PDB-CPX-530841 (preferred)
Entry contents:
33 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (35 distinct):
Small ribosomal subunit protein uS2 Chain: A
Molecule details ›
Chain: A
Length: 295 amino acids
Theoretical weight: 32.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P08865 (Residues: 1-295; Coverage: 100%)
Gene names: LAMBR, LAMR1, RPSA
Sequence domains:
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1
Small ribosomal subunit protein eS1 Chain: B
Molecule details ›
Chain: B
Length: 264 amino acids
Theoretical weight: 30 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P61247 (Residues: 1-264; Coverage: 100%)
Gene names: FTE1, MFTL, RPS3A
Sequence domains: Ribosomal S3Ae family
Small ribosomal subunit protein uS5 Chain: C
Molecule details ›
Chain: C
Length: 293 amino acids
Theoretical weight: 31.38 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P15880 (Residues: 1-293; Coverage: 100%)
Gene names: RPS2, RPS4
Sequence domains:
Structure domains:
Small ribosomal subunit protein uS3 Chain: D
Molecule details ›
Chain: D
Length: 243 amino acids
Theoretical weight: 26.73 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P23396 (Residues: 1-243; Coverage: 100%)
Gene names: OK/SW-cl.26, RPS3
Sequence domains:
Structure domains:
Small ribosomal subunit protein eS4, Y isoform 1 Chain: E
Molecule details ›
Chain: E
Length: 263 amino acids
Theoretical weight: 29.51 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P22090 (Residues: 1-263; Coverage: 100%)
Gene names: PRO2646, RPS4Y, RPS4Y1
Sequence domains:
Structure domains:
Small ribosomal subunit protein uS7 Chain: F
Molecule details ›
Chain: F
Length: 204 amino acids
Theoretical weight: 22.91 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P46782 (Residues: 1-204; Coverage: 100%)
Gene name: RPS5
Sequence domains: Ribosomal protein S7p/S5e
Structure domains: Ribosomal protein S7 domain
Small ribosomal subunit protein eS6 Chain: G
Molecule details ›
Chain: G
Length: 249 amino acids
Theoretical weight: 28.75 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62753 (Residues: 1-249; Coverage: 100%)
Gene names: OK/SW-cl.2, RPS6
Sequence domains: Ribosomal protein S6e
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Small ribosomal subunit protein eS7 Chain: H
Molecule details ›
Chain: H
Length: 194 amino acids
Theoretical weight: 22.17 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62081 (Residues: 1-194; Coverage: 100%)
Gene name: RPS7
Sequence domains: Ribosomal protein S7e
Small ribosomal subunit protein eS8 Chain: I
Molecule details ›
Chain: I
Length: 208 amino acids
Theoretical weight: 24.26 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62241 (Residues: 1-208; Coverage: 100%)
Gene names: OK/SW-cl.83, RPS8
Sequence domains: Ribosomal protein S8e
Structure domains: Ribosomal protein S8e, subdomain
Small ribosomal subunit protein uS4 Chain: J
Molecule details ›
Chain: J
Length: 194 amino acids
Theoretical weight: 22.64 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P46781 (Residues: 1-194; Coverage: 100%)
Gene name: RPS9
Sequence domains:
Small ribosomal subunit protein eS10 Chain: K
Molecule details ›
Chain: K
Length: 165 amino acids
Theoretical weight: 18.93 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P46783 (Residues: 1-165; Coverage: 100%)
Gene name: RPS10
Sequence domains: Plectin/S10 domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Small ribosomal subunit protein uS17 Chain: L
Molecule details ›
Chain: L
Length: 158 amino acids
Theoretical weight: 18.47 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62280 (Residues: 1-158; Coverage: 100%)
Gene name: RPS11
Sequence domains:
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Small ribosomal subunit protein eS12 Chain: M
Molecule details ›
Chain: M
Length: 132 amino acids
Theoretical weight: 14.54 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P25398 (Residues: 1-132; Coverage: 100%)
Gene name: RPS12
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
Small ribosomal subunit protein uS15 Chain: N
Molecule details ›
Chain: N
Length: 151 amino acids
Theoretical weight: 17.26 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62277 (Residues: 1-151; Coverage: 100%)
Gene name: RPS13
Sequence domains:
Structure domains: S15/NS1, RNA-binding
Small ribosomal subunit protein uS11 Chain: O
Molecule details ›
Chain: O
Length: 151 amino acids
Theoretical weight: 16.3 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62263 (Residues: 1-151; Coverage: 100%)
Gene names: PRO2640, RPS14
Sequence domains: Ribosomal protein S11
Structure domains: Ribosomal protein S11
Small ribosomal subunit protein uS19 Chain: P
Molecule details ›
Chain: P
Length: 145 amino acids
Theoretical weight: 17.08 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62841 (Residues: 1-145; Coverage: 100%)
Gene names: RIG, RPS15
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein uS9 Chain: Q
Molecule details ›
Chain: Q
Length: 146 amino acids
Theoretical weight: 16.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62249 (Residues: 1-146; Coverage: 100%)
Gene name: RPS16
Sequence domains: Ribosomal protein S9/S16
Structure domains: Ribosomal Protein S5; domain 2
Small ribosomal subunit protein eS17 Chain: R
Molecule details ›
Chain: R
Length: 135 amino acids
Theoretical weight: 15.58 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P08708 (Residues: 1-135; Coverage: 100%)
Gene names: RPS17, RPS17L
Sequence domains: Ribosomal S17
Structure domains: Ribosomal protein S17e-like
Small ribosomal subunit protein uS13 Chain: S
Molecule details ›
Chain: S
Length: 152 amino acids
Theoretical weight: 17.76 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62269 (Residues: 1-152; Coverage: 100%)
Gene names: D6S218E, RPS18
Sequence domains: Ribosomal protein S13/S18
Structure domains: Helicase, Ruva Protein; domain 3
Small ribosomal subunit protein eS19 Chain: T
Molecule details ›
Chain: T
Length: 145 amino acids
Theoretical weight: 16.09 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P39019 (Residues: 1-145; Coverage: 100%)
Gene name: RPS19
Sequence domains: Ribosomal protein S19e
Small ribosomal subunit protein uS10 Chain: U
Molecule details ›
Chain: U
Length: 119 amino acids
Theoretical weight: 13.4 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P60866 (Residues: 1-119; Coverage: 100%)
Gene name: RPS20
Sequence domains: Ribosomal protein S10p/S20e
Structure domains: Ribosomal protein S10 domain
Small ribosomal subunit protein eS21 Chain: V
Molecule details ›
Chain: V
Length: 83 amino acids
Theoretical weight: 9.12 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P63220 (Residues: 1-83; Coverage: 100%)
Gene name: RPS21
Sequence domains: Ribosomal protein S21e
Structure domains: Hypothetical Cytosolic Protein; Chain: A;
Small ribosomal subunit protein uS8 Chain: W
Molecule details ›
Chain: W
Length: 130 amino acids
Theoretical weight: 14.87 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62244 (Residues: 1-130; Coverage: 100%)
Gene names: OK/SW-cl.82, RPS15A
Sequence domains: Ribosomal protein S8
Structure domains:
Small ribosomal subunit protein uS12 Chain: X
Molecule details ›
Chain: X
Length: 143 amino acids
Theoretical weight: 15.84 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62266 (Residues: 1-143; Coverage: 100%)
Gene name: RPS23
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein eS24 Chain: Y
Molecule details ›
Chain: Y
Length: 133 amino acids
Theoretical weight: 15.46 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62847 (Residues: 1-133; Coverage: 100%)
Gene name: RPS24
Sequence domains: Ribosomal protein S24e
Structure domains: Alpha-Beta Plaits
Small ribosomal subunit protein eS25 Chain: Z
Molecule details ›
Chain: Z
Length: 125 amino acids
Theoretical weight: 13.78 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62851 (Residues: 1-125; Coverage: 100%)
Gene name: RPS25
Sequence domains: S25 ribosomal protein
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Small ribosomal subunit protein eS26 Chain: a
Molecule details ›
Chain: a
Length: 115 amino acids
Theoretical weight: 13.05 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62854 (Residues: 1-115; Coverage: 100%)
Gene name: RPS26
Sequence domains: Ribosomal protein S26e
Structure domains: Ribosomal protein S26e
Small ribosomal subunit protein eS27 Chain: b
Molecule details ›
Chain: b
Length: 84 amino acids
Theoretical weight: 9.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P42677 (Residues: 1-84; Coverage: 100%)
Gene names: MPS1, RPS27
Sequence domains: Ribosomal protein S27
Structure domains: Zn-binding ribosomal proteins
Small ribosomal subunit protein eS28 Chain: c
Molecule details ›
Chain: c
Length: 69 amino acids
Theoretical weight: 7.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62857 (Residues: 1-69; Coverage: 100%)
Gene name: RPS28
Sequence domains: Ribosomal protein S28e
Structure domains: Nucleic acid-binding proteins
Small ribosomal subunit protein uS14 Chain: d
Molecule details ›
Chain: d
Length: 56 amino acids
Theoretical weight: 6.69 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62273 (Residues: 1-56; Coverage: 100%)
Gene name: RPS29
Sequence domains: Ribosomal protein S14p/S29e
Structure domains: 30s Ribosomal Protein S14; Chain N
Small ribosomal subunit protein eS30 Chain: e
Molecule details ›
Chain: e
Length: 59 amino acids
Theoretical weight: 6.67 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62861 (Residues: 75-133; Coverage: 44%)
Gene name: FAU
Sequence domains: Ribosomal protein S30
Ubiquitin-ribosomal protein eS31 fusion protein Chain: f
Molecule details ›
Chain: f
Length: 156 amino acids
Theoretical weight: 18 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62979 (Residues: 1-156; Coverage: 100%)
Gene names: RPS27A, UBA80, UBCEP1
Sequence domains:
Structure domains: N-terminal domain of TfIIb
Small ribosomal subunit protein RACK1 Chain: g
Molecule details ›
Chain: g
Length: 317 amino acids
Theoretical weight: 35.12 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P63244 (Residues: 1-317; Coverage: 100%)
Gene names: GNB2L1, HLC7, PIG21, RACK1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
18S Ribosomal RNA Chain: 1
Molecule details ›
Chain: 1
Length: 1869 nucleotides
Theoretical weight: 602.78 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA
HCV-IRES Chain: z
Molecule details ›
Chain: z
Length: 504 nucleotides
Theoretical weight: 162.19 KDa
Sequence domains:
  • Hepatitis C virus internal ribosome entry site
  • Hepatitis C alternative reading frame stem-loop

    • Ligands and Environments

    2 bound ligands:
    Ligand MG 74 x MG
    Ligand ZN 2 x ZN
    No modified residues

    Experiments and Validation Details

    Entry percentile scores
    Resolution: 3.9Å
    Relevant EMDB volumes: EMD-3221

    Quick links

    Citations

    22 review citations

    The Emerging Role of RNA as a Therapeutic Target for Small Molecules.
    Connelly et al. (2016)
    21 more

    6 mentions without citation

    Dynamics of IRES-mediated translation.
    Johnson et al. (2017)
    5 more