› 5fmp

X-ray diffraction
2.26Å resolution

KstR, transcriptional repressor of cholesterol degradation in Mycobacterium tuberculosis, bound to the DNA operator

Released:
DOI: 10.2210/pdb5fmp/pdb
PDB entry 5fmp coloured by chain, front view.
PDB entry 5fmp coloured by chain, side view.
PDB entry 5fmp coloured by chain, top view.
Source organisms:
Entry authors: Podust LM, Ouellet H

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118684 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH-type transcriptional repressor KstR Chains: A, B
Molecule details ›
Chains: A, B
Length: 205 amino acids
Theoretical weight: 22.83 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P96856 (Residues: 23-220; Coverage: 90%)
Gene names: Rv3574, kstR
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2
5'-D(*CP*TP*AP*GP*AP*AP*CP*GP*TP*GP*TP*TP*CP*TP*AP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Not provided
5'-D(*TP*TP*AP*GP*AP*AP*CP*AP*CP*GP*TP*TP*CP*TP*AP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 80.53Å b: 62.781Å c: 98.647Å
α: 90° β: 89.9° γ: 90°
R-values:
R R work R free
0.205 0.202 0.27
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links