› 5fs5

X-ray diffraction
1.42Å resolution

Breaking down the wall: mutation of the tyrosine gate of the universal Escherichia coli fimbrial adhesin FimH

Released:
DOI: 10.2210/pdb5fs5/pdb
PDB entry 5fs5 coloured by chain, front view.
PDB entry 5fs5 coloured by chain, side view.
PDB entry 5fs5 coloured by chain, top view.
Source organism: Escherichia coli UTI89
Primary publication:
Mutation of Tyr137 of the universal Escherichia coli fimbrial adhesin FimH relaxes the tyrosine gate prior to mannose binding.
Rabbani S, Krammer EM, Roos G, Zalewski A, Preston R, Eid S, Zihlmann P, Prévost M, Lensink MF, Thompson A, Ernst B, Bouckaert J
OpenAccess logo IUCrJ 4 7-23 (2017)
PMID: 28250938

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140004 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type 1 fimbrin D-mannose specific adhesin Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 16.82 KDa
Source organism: Escherichia coli UTI89
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08191 (Residues: 22-179; Coverage: 57%)
Gene names: JW4283, b4320, fimH
Sequence domains: FimH, mannose binding
Structure domains: Fimbrial-type adhesion domain

Ligands and Environments

2 bound ligands:
Ligand KGM 1 x KGM
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21212
Unit cell:
a: 59.565Å b: 96.917Å c: 22.999Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.108 0.106 0.16
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

FimH and Anti-Adhesive Therapeutics: A Disarming Strategy Against Uropathogens.
Sarshar et al. (2020)
2 more

1 mention without citation

Mutation of Tyr137 of the universal Escherichia coli fimbrial adhesin FimH relaxes the tyrosine gate prior to mannose binding.
Rabbani et al. (2017)