› 5fu2

X-ray diffraction
1.4Å resolution

The complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition

Released:
DOI: 10.2210/pdb5fu2/pdb
PDB entry 5fu2 coloured by chain, front view.
PDB entry 5fu2 coloured by chain, side view.
PDB entry 5fu2 coloured by chain, top view.
Source organism: Ruminococcus flavefaciens
Primary publication:
Complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition.
Venditto I, Luis AS, Rydahl M, Schückel J, Fernandes VO, Vidal-Melgosa S, Bule P, Goyal A, Pires VM, Dourado CG, Ferreira LM, Coutinho PM, Henrissat B, Knox JP, Baslé A, Najmudin S, Gilbert HJ, Willats WG, Fontes CM
Proc Natl Acad Sci U S A 113 7136-41 (2016)
PMID: 27298375

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-495408 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Carbohydrate binding module 74 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 11.83 KDa
Source organism: Ruminococcus flavefaciens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1A9TAF4 (Residues: 1-106; Coverage: 100%)
Sequence domains: Carbohydrate binding module-like

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand CA 1 x CA
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 45.623Å b: 47.272Å c: 83.958Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Advances in molecular engineering of carbohydrate-binding modules.
Armenta et al. (2017)
7 more

1 mention without citation

Complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition.
Venditto et al. (2016)