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RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments.

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Nurizzo D, Bowler MW, Caserotto H, Dobias F, Giraud T, Surr J, Guichard N, Papp G, Guijarro M, Mueller-Dieckmann C, Flot D, McSweeney S, Cipriani F, Theveneau P, Leonard GA
OpenAccess logo Acta Crystallogr D Struct Biol 72 966-75 (2016)
Related entries: 5fxm, 5fxn

Cited: 15 times
EuropePMC logo PMID: 27487827

Abstract

Automation of the mounting of cryocooled samples is now a feature of the majority of beamlines dedicated to macromolecular crystallography (MX). Robotic sample changers have been developed over many years, with the latest designs increasing capacity, reliability and speed. Here, the development of a new sample changer deployed at the ESRF beamline MASSIF-1 (ID30A-1), based on an industrial six-axis robot, is described. The device, named RoboDiff, includes a high-capacity dewar, acts as both a sample changer and a high-accuracy goniometer, and has been designed for completely unattended sample mounting and diffraction data collection. This aim has been achieved using a high level of diagnostics at all steps of the process from mounting and characterization to data collection. The RoboDiff has been in service on the fully automated endstation MASSIF-1 at the ESRF since September 2014 and, at the time of writing, has processed more than 20 000 samples completely automatically.

Articles - 5fxl mentioned but not cited (2)

  1. RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments. Nurizzo D, Bowler MW, Caserotto H, Dobias F, Giraud T, Surr J, Guichard N, Papp G, Guijarro M, Mueller-Dieckmann C, Flot D, McSweeney S, Cipriani F, Theveneau P, Leonard GA. Acta Crystallogr D Struct Biol 72 966-975 (2016)
  2. Quantifying and comparing radiation damage in the Protein Data Bank. Shelley KL, Garman EF. Nat Commun 13 1314 (2022)


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  1. BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions. Bery N, Cruz-Migoni A, Bataille CJ, Quevedo CE, Tulmin H, Miller A, Russell A, Phillips SE, Carr SB, Rabbitts TH. Elife 7 e37122 (2018)
  2. Epsin and Sla2 form assemblies through phospholipid interfaces. Garcia-Alai MM, Heidemann J, Skruzny M, Gieras A, Mertens HDT, Svergun DI, Kaksonen M, Uetrecht C, Meijers R. Nat Commun 9 328 (2018)
  3. Multi-position data collection and dynamic beam sizing: recent improvements to the automatic data-collection algorithms on MASSIF-1. Svensson O, Gilski M, Nurizzo D, Bowler MW. Acta Crystallogr D Struct Biol 74 433-440 (2018)
  4. The TELL automatic sample changer for macromolecular crystallography. Martiel I, Buntschu D, Meier N, Gobbo A, Panepucci E, Schneider R, Heimgartner P, Müller D, Bühlmann K, Birri M, Kaminski JW, Leuenberger J, Oliéric V, Glettig W, Wang M. J Synchrotron Radiat 27 860-863 (2020)
  5. FragMAXapp: crystallographic fragment-screening data-analysis and project-management system. Lima GMA, Jagudin E, Talibov VO, Benz LS, Marullo C, Barthel T, Wollenhaupt J, Weiss MS, Mueller U. Acta Crystallogr D Struct Biol 77 799-808 (2021)
  6. Robotic sample changers for macromolecular X-ray crystallography and biological small-angle X-ray scattering at the National Synchrotron Light Source II. Lazo EO, Antonelli S, Aishima J, Bernstein HJ, Bhogadi D, Fuchs MR, Guichard N, McSweeney S, Myers S, Qian K, Schneider D, Shea-McCarthy G, Skinner J, Sweet R, Yang L, Jakoncic J. J Synchrotron Radiat 28 1649-1661 (2021)
  7. Orthorhombic lysozyme crystallization at acidic pH values driven by phosphate binding. Plaza-Garrido M, Salinas-Garcia MC, Camara-Artigas A. Acta Crystallogr D Struct Biol 74 480-489 (2018)
  8. Structural basis for the bi-functionality of human oxaloacetate decarboxylase FAHD1. Weiss AKH, Naschberger A, Loeffler JR, Gstach H, Bowler MW, Holzknecht M, Cappuccio E, Pittl A, Etemad S, Dunzendorfer-Matt T, Scheffzek K, Liedl KR, Jansen-Dürr P. Biochem J 475 3561-3576 (2018)
  9. Raoult's law revisited: accurately predicting equilibrium relative humidity points for humidity control experiments. Bowler MG, Bowler DR, Bowler MW. J Appl Crystallogr 50 631-638 (2017)
  10. Structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse. Weiss AKH, Naschberger A, Cappuccio E, Metzger C, Mottes L, Holzknecht M, von Velsen J, Bowler MW, Rupp B, Jansen-Dürr P. Biosci Rep 40 BSR20194431 (2020)
  11. Unravelling the Adaptation Mechanisms to High Pressure in Proteins. Caliò A, Dubois C, Fontanay S, Koza MM, Hoh F, Roumestand C, Oger P, Peters J. Int J Mol Sci 23 8469 (2022)
  12. Elucidating polymorphs of crystal structures by intensity-based hierarchical clustering analysis of multiple diffraction data sets. Matsuura H, Sakai N, Toma-Fukai S, Muraki N, Hayama K, Kamikubo H, Aono S, Kawano Y, Yamamoto M, Hirata K. Acta Crystallogr D Struct Biol 79 909-924 (2023)
  13. Structural Analyses of Bacterial Effectors by X-Ray Crystallography. Dugelay C, Gueguen-Chaignon V, Terradot L. Methods Mol Biol 2715 485-502 (2024)


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