› 5fyf

X-ray diffraction
2.04Å resolution

Structure of CYP153A from Marinobacter aquaeolei

Released:
DOI: 10.2210/pdb5fyf/pdb
PDB entry 5fyf coloured by chain, front view.
PDB entry 5fyf coloured by chain, side view.
PDB entry 5fyf coloured by chain, top view.
Source organism: Marinobacter nauticus
Primary publication:
Structure-Guided Redesign of CYP153AM.aqfor the Improved Terminal Hydroxylation of Fatty Acids
Hoffman SM, Danesh Azari H-R, Spandolf C, Weissenborn M, Grogan G, Hauer B
ChemCatChem (2016)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106712 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chains: A, B
Molecule details ›
Chains: A, B
Length: 470 amino acids
Theoretical weight: 54.02 KDa
Source organism: Marinobacter nauticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1TY82 (Residues: 1-470; Coverage: 100%)
Gene name: Maqu_0600
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 59.59Å b: 71.11Å c: 215.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links