5fyw

Electron Microscopy
4.35Å resolution

Transcription initiation complex structures elucidate DNA opening (OC)

Released:
Source organism: Saccharomyces cerevisiae
Related structures: EMD-3378

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
PDBe Complex ID:
PDB-CPX-118693 (preferred)
Entry contents:
20 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (22 distinct):
DNA-directed RNA polymerase II subunit RPB1 Chain: A
DNA-directed RNA polymerase II subunit RPB2 Chain: B
Molecule details ›
Chain: B
Length: 1224 amino acids
Theoretical weight: 138.94 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P08518 (Residues: 1-1224; Coverage: 100%)
Gene names: RPB150, RPB2, RPO22, YOR151C
Sequence domains:
DNA-directed RNA polymerase II subunit RPB3 Chain: C
DNA-directed RNA polymerase II subunit RPB4 Chain: D
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
DNA-directed RNA polymerase II subunit RPB7 Chain: G
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
DNA-directed RNA polymerase II subunit RPB9 Chain: I
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
DNA-directed RNA polymerase II subunit RPB11 Chain: K
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: L
Transcription initiation factor IIB Chain: M
TATA-box-binding protein Chain: O
Transcription initiation factor IIF subunit alpha Chain: Q
Transcription initiation factor IIF subunit beta Chain: R
Transcription initiation factor IIA large subunit Chain: U
Transcription initiation factor IIA subunit 2 Chain: V
Transcription initiation factor IIE subunit alpha Chain: W
Transcription initiation factor IIE subunit beta Chain: X
NONTEMPLATE DNA Chain: N
NONTEMPLATE DNA Chain: T

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 4.35Å
Relevant EMDB volumes: EMD-3378
Expression system: Escherichia coli BL21(DE3)