› 5g04

Electron Microscopy
3.9Å resolution

Structure of the human APC-Cdc20-Hsl1 complex

Released:
DOI: 10.2210/pdb5g04/pdb
PDB entry 5g04 coloured by chain, front view.
PDB entry 5g04 coloured by chain, side view.
PDB entry 5g04 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular mechanism of APC/C activation by mitotic phosphorylation.
Zhang S, Chang L, Alfieri C, Zhang Z, Yang J, Maslen S, Skehel M, Barford D
Nature 533 260-264 (2016)
PMID: 27120157
Related structures: EMD-3385

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
PDBe Complex ID:
PDB-CPX-151686 (preferred)
Entry contents:
16 distinct polypeptide molecules
Macromolecules (16 distinct):
Anaphase-promoting complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 1944 amino acids
Theoretical weight: 216.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9H1A4 (Residues: 1-1944; Coverage: 100%)
Gene names: ANAPC1, TSG24
Sequence domains:
Anaphase-promoting complex subunit 11 Chain: B
Molecule details ›
Chain: B
Length: 84 amino acids
Theoretical weight: 9.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NYG5 (Residues: 1-84; Coverage: 100%)
Gene names: ANAPC11, HSPC214
Sequence domains: Anaphase-promoting complex subunit 11 RING-H2 finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Cell division cycle protein 23 homolog Chains: C, P
Anaphase-promoting complex subunit 15 Chain: D
Anaphase-promoting complex subunit 16 Chain: E
Cell division cycle protein 27 homolog Chains: F, H
Anaphase-promoting complex subunit CDC26 Chains: G, W
Anaphase-promoting complex subunit 4 Chain: I
Cell division cycle protein 16 homolog Chains: J, K
Anaphase-promoting complex subunit 10 Chain: L
Anaphase-promoting complex subunit 13 Chain: M
Anaphase-promoting complex subunit 2 Chain: N
Anaphase-promoting complex subunit 5 Chain: O
Cell division cycle protein 20 homolog Chain: R
Probable serine/threonine-protein kinase HSL1 Chain: S
Anaphase-promoting complex subunit 7 Chains: X, Y

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-3385
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

23 review citations

Ubiquitin Ligases: Structure, Function, and Regulation.
Zheng et al. (2017)
22 more

8 mentions without citation

Visualizing the complex functions and mechanisms of the anaphase promoting complex/cyclosome (APC/C).
Alfieri et al. (2017)
7 more