5g2x

Electron Microscopy
3.8Å resolution

Structure a of Group II Intron Complexed with its Reverse Transcriptase

Released:
Related structures: EMD-3331

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-141222 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Group II intron-encoded protein LtrA Chain: C
Molecule details ›
Chain: C
Length: 599 amino acids
Theoretical weight: 70.29 KDa
Source organism: Lactococcus cremoris
Expression system: Lactococcus lactis
UniProt:
  • Canonical: P0A3U0 (Residues: 1-599; Coverage: 100%)
Gene names: ltrA, matR
Sequence domains:
GROUP II INTRON Chain: A
Molecule details ›
Chain: A
Length: 704 nucleotides
Theoretical weight: 227.63 KDa
Source organism: Lactococcus lactis
Expression system: Lactococcus lactis
Sequence domains: Group II catalytic intron
5'-R(*CP*AP*CP*AP*UP*CP*CP*AP*UP*AP*AP*CP)-3' Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.74 KDa
Source organism: Lactococcus cremoris
Expression system: Lactococcus lactis

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-3331
Expression system: Lactococcus lactis