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Structure of a group II intron in complex with its reverse transcriptase.

<div class='caption-title'>Overall Architecture of Group II Intron RNP Complex</div>
<div class='caption-title'>Structures of the Group II Introns</div>
<div class='caption-title'>Structure Model of LtrA</div>
Qu G, Kaushal PS, Wang J, Shigematsu H, Piazza CL, Agrawal RK, Belfort M, Wang HW
OpenAccess logo Nat Struct Mol Biol 23 549-57 (2016)
Cited: 69 times
EuropePMC logo PMID: 27136327

Abstract

Bacterial group II introns are large catalytic RNAs related to nuclear spliceosomal introns and eukaryotic retrotransposons. They self-splice, yielding mature RNA, and integrate into DNA as retroelements. A fully active group II intron forms a ribonucleoprotein complex comprising the intron ribozyme and an intron-encoded protein that performs multiple activities including reverse transcription, in which intron RNA is copied into the DNA target. Here we report cryo-EM structures of an endogenously spliced Lactococcus lactis group IIA intron in its ribonucleoprotein complex form at 3.8-Å resolution and in its protein-depleted form at 4.5-Å resolution, revealing functional coordination of the intron RNA with the protein. Remarkably, the protein structure reveals a close relationship between the reverse transcriptase catalytic domain and telomerase, whereas the active splicing center resembles the spliceosomal Prp8 protein. These extraordinary similarities hint at intricate ancestral relationships and provide new insights into splicing and retromobility.

Reviews - 5g2y mentioned but not cited (2)

  1. Unraveling the structure and biological functions of RNA triple helices. Brown JA. Wiley Interdiscip Rev RNA 11 e1598 (2020)
  2. Structural Insights into the Mechanism of Group II Intron Splicing. Zhao C, Pyle AM. Trends Biochem Sci 42 470-482 (2017)

Articles - 5g2y mentioned but not cited (1)

  1. An ultraprocessive, accurate reverse transcriptase encoded by a metazoan group II intron. Zhao C, Liu F, Pyle AM. RNA 24 183-195 (2018)


Reviews citing this publication (20)

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Articles citing this publication (46)

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